Author(s): Manish Kumar, Sumit Prakash Yadav, Pooja and Krishan Yadav

Abstract: The aim of study was to examine the efficiency of Egg yolk-tris-fructose extender (EYTF) and Egg yolk-tris-fructose extender (EYTF) +100μg/ml ethanolic extract of Ram Tulsi (Ocimum gratissimum) in preserving semen of dogs (n=6) at different time slots (0, 24, 48, and 72 hours) at refrigeration temperature (4 °C). Total 24 semen ejaculates were collected by digital manipulation at weekly intervals. The fresh semen was examined for macroscopic (volume, colour, consistency and pH) and microscopic parameters (mass motility, individual motility, sperm concentration, sperm abnormalities, viability and HOST) immediately after collection. Extended samples were evaluated for individual sperm motility, viability, ABNORMALITIES, and sperm function test (HOST). The average individual sperm motility, live sperm percentage and HOST were reduced significantly (p<0.05) in Egg yolk-tris-fructose extender (EYTF) and Egg yolk-tris-fructose extender (EYTF) +100 μg/ml ethanolic extract of Ram Tulsi (Ocimum gratissimum) from 0 to 72 hours. When individual sperm motility, abnormal spermatozoa and HOST percentage was compared between Egg yolk-tris-fructose extender (EYTF) and Egg yolk-tris-fructose extender (EYTF) +100 μg/ml ethanolic extract of Ram Tulsi (Ocimum gratissimum) at 0 hour and 24 hour there was non-significant difference but at 48- and 72-hours significant difference was observed. The percentage of live spermatozoa differed significantly at 24, 48, and 72 hours (p<0.05) between extenders. The percentage of abnormal spermatozoa significantly increased from 0 to 72 hours of preservation both in Egg yolk-tris-fructose extender (EYTF) and Egg yolk-tris-fructose extender (EYTF) +100 μg/ml ethanolic extract of Ram Tulsi (Ocimum gratissimum). In conclusion, the Egg yolk-tris-fructose extender (EYTF) +100 μg/ml ethanolic extract of Ram Tulsi (Ocimum gratissimum) was found better than Egg yolk-tris-fructose extender (EYTF) the extender in terms of preserving the motility, viability, and membrane integrity of refrigerated canine semen for up to 72 hours, suggesting it a simple, economical, and efficient extender for canine semen refrigeration.

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