Author(s): Pulin Kerketta

Abstract: Recently, antibacterial resistance is the one of the major problems faced by the people. The need of the hour is to obtain natural biopolymer with antibacterial efficacy. In the current study, chitosan was extracted from the shell of freshwater prawn, M. dayanum. M. dayanum are edible prawns, found in ponds and rivers of Jharkhand. The extracted chitosan was structurally characterized and degree of deacetylation was calculated through FT-IR spectroscopy. Further the antibacterial activity of chitosan was assayed by Minimum Inhibitory Concentration (MIC). The result of the present study showed that FT-IR spectrum of extracted chitosan contains peak at 898,1033,1419,1554,1651, 2881 and 3263 cm^-1. The degree of deacetylation of the extracted chitosan was calculated as 79.37±0.58%. The result revealed that in case of E. coli (ATCC 8739), MIC of chitosan was 0.15 mg/ml and percentage of bacterial growth was 75.86% and percentage of minimum bacterial growth inhibition was 24.14%. In case of P. aeruginosa (ATCC 10145) MIC of chitosan was 0.15 mg/ml and percentage of bacterial growth was 95.68% and percentage of minimum bacterial growth inhibition was 4.32%. Statistical analysis showed that the MIC value of chitosan concentration of 0.15 mg/ml obtained from M. dayanum against E.coli (ATCC 8739) was significantly more susceptible than against P. aeruginosa (ATCC 10145) with MIC value of 0.15 mg/ml (p<0.001) based on the O.D observed. The assay showed that chitosan has concentration dependent antibacterial activity against human pathogenic bacterial strains which designates its probable use as effective antibacterial agent.

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