Author(s): AR Jadhav, SJ Waghmare, MS Kambale and VS Patil

Abstract: The rhizosphere is a dynamic region governed by complex interactions between plants and the organisms that are in close association with the root. Azospirillum is one of the versatile non-symbiotic, free living diazotrophic bacteria which appears to have a world-wide distribution and occurs in large number in the rhizosphere soil of a variety of grasses and cereals. There are various types of soil microbes which can solubilise the fixed form of P and make it available to plants. Twenty root and rhizospheric soil samples were collected from different tehsils of Kolhapur District. Total seven Azospirillum and eight Phosphate solubilizing bacteria (PSB) isolates were obtained. All Azospirillum and PSB isolates were identified on the basis of morphological, microscopic features and different biochemical tests. Biochemical tests were studied viz. methyl red test, catalase test, starch hydrolyse test, gelatine hydrolysis test, gas production, H2S production, indol test, and nitrate reductase test. Most of isolates showed positive while some showed negative test. All the isolates of Azospirillum were gram negative and had white subsurface pellicle on semi solid NFB media. All the isolates were rod shaped except the isolate 5 which was vibroid shaped. All the eight Phosphate solubilizing bacterial isolates had white, smooth colonies. Some showed irregular colonies whereas some showed circular colonies. All isolates were gram negative.

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